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Procedure price report · Laboratory - Chemistry and Hematology

Immunoassay for one substance, multiple-step method

CPT 83516the billing code this page is about

An antibody-based lab test that detects or roughly measures a single non-infectious substance using a multiple-step laboratory method; billed once per substance tested.

Hospitals typically charge $38 cash for this outpatient procedure — the median across 3,356 hospitals that filed a usable cash price; the average of the middle 60% is $42. Most fall between $14 and $92 (20th–80th percentile).

Hospitals
3,356
States
50
Filings dated
Aug 2022 – Sep 2026
Computed
September 25, 2026
Prices checked
through September 25, 2026

Median: half of hospitals filed less, half more — and it is a price some hospital actually filed. Average of the middle 60%: the mean of the prices between the 20th and 80th percentiles. We never publish the plain average, the lowest or the highest price: the extremes in hospital price files are chargemaster artefacts, not prices anyone pays.

What hospitals charge, from cheapest to most expensive

Cash price percentiles, outpatient, across 3,356 hospitals. Each figure is a price a hospital filed. 10th: one in ten hospitals charges less; 25th: a quarter charge less; Median: half charge less; 75th: three quarters charge less; 90th: nine in ten charge less.
10th25thMedian75th90th
$10$18$38$76$134

The most expensive tenth of hospitals charges at least 13× what the cheapest tenth charges ($10 vs $134).

How the prices are spread

$7 – $10: 178 hospitals$7$10 – $14: 312 hospitals$14 – $20: 285 hospitals$14$20 – $27: 354 hospitals$27 – $38: 369 hospitals$27$38 – $52: 373 hospitals$52 – $72: 414 hospitals$52$72 – $99: 321 hospitals$99 – $138: 266 hospitals$99$138 – $190: 150 hospitals$138median $38price bands are equal on a log scale
Hospitals per price band (3,022 of 3,356 — the bands cover the 5th to 95th percentile)
FromToHospitals
$7$10178
$10$14312
$14$20285
$20$27354
$27$38369
$38$52373
$52$72414
$72$99321
$99$138266
$138$190150

By state

The median cash price in each state, against the national median of $38.

Alaska: median $110 across 19 hospitalsAK$110Maine: median $57 across 28 hospitalsME$57Vermont: median $95 across 14 hospitalsVT$95New Hampshire: median $53 across 24 hospitalsNH$53Washington: median $52 across 77 hospitalsWA$52Idaho: median $41 across 24 hospitalsID$41Montana: median $36 across 33 hospitalsMT$36North Dakota: median $68 across 33 hospitalsND$68Minnesota: median $54 across 106 hospitalsMN$54Illinois: median $47 across 143 hospitalsIL$47Wisconsin: median $47 across 106 hospitalsWI$47Michigan: median $16 across 101 hospitalsMI$16New York: median $28 across 118 hospitalsNY$28Massachusetts: median $56 across 52 hospitalsMA$56Oregon: median $44 across 49 hospitalsOR$44Nevada: median $57 across 20 hospitalsNV$57Wyoming: median $39 across 16 hospitalsWY$39South Dakota: median $64 across 38 hospitalsSD$64Iowa: median $64 across 90 hospitalsIA$64Indiana: median $30 across 96 hospitalsIN$30Ohio: median $37 across 141 hospitalsOH$37Pennsylvania: median $48 across 120 hospitalsPA$48New Jersey: median $13 across 57 hospitalsNJ$13Connecticut: median $35 across 20 hospitalsCT$35Rhode Island: median $46 across 10 hospitalsRI$46California: median $16 across 148 hospitalsCA$16Utah: median $17 across 43 hospitalsUT$17Colorado: median $63 across 69 hospitalsCO$63Nebraska: median $59 across 81 hospitalsNE$59Missouri: median $28 across 76 hospitalsMO$28Kentucky: median $32 across 69 hospitalsKY$32West Virginia: median $22 across 44 hospitalsWV$22Virginia: median $52 across 73 hospitalsVA$52Maryland: median $66 across 41 hospitalsMD$66Delaware: 7 hospitals filed a usable price — fewer than 10, so no figureDEArizona: median $64 across 42 hospitalsAZ$64New Mexico: median $100 across 29 hospitalsNM$100Kansas: median $57 across 91 hospitalsKS$57Arkansas: median $21 across 37 hospitalsAR$21Tennessee: median $30 across 81 hospitalsTN$30North Carolina: median $28 across 89 hospitalsNC$28South Carolina: median $81 across 53 hospitalsSC$81Oklahoma: median $20 across 74 hospitalsOK$20Louisiana: median $26 across 49 hospitalsLA$26Mississippi: median $14 across 57 hospitalsMS$14Alabama: median $38 across 45 hospitalsAL$38Georgia: median $44 across 109 hospitalsGA$44Hawaii: median $58 across 18 hospitalsHI$58Texas: median $52 across 244 hospitalsTX$52Florida: median $15 across 152 hospitalsFL$15
Cash price by state, most expensive first. National median $38. A state needs at least 10 hospitals with a usable price to show a figure.
StateHospitalsMedianvs national20th–80th
Alaska AK19$110+189%$18 – $209
New Mexico NM29$100+163%$37 – $199
Vermont VT14$95+149%$31 – $209
South Carolina SC53$81+112%$25 – $114
North Dakota ND33$68+79%$20 – $94
Maryland MD41$66+74%$52 – $81
Iowa IA90$64+69%$29 – $98
South Dakota SD38$64+68%$38 – $98
Arizona AZ42$64+68%$14 – $143
Colorado CO69$63+66%$22 – $81
Nebraska NE81$59+55%$13 – $162
Hawaii HI18$58+53%$14 – $108
Maine ME28$57+51%$30 – $80
Kansas KS91$57+50%$25 – $92
Nevada NV20$57+49%$17 – $113
Massachusetts MA52$56+48%$30 – $113
Minnesota MN106$54+43%$16 – $94
New Hampshire NH24$53+38%$26 – $103
Washington WA77$52+37%$24 – $101
Virginia VA73$52+37%$16 – $96
Texas TX244$52+36%$20 – $118
Pennsylvania PA120$48+26%$13 – $102
Illinois IL143$47+25%$23 – $141
Wisconsin WI106$47+24%$15 – $88
Rhode Island RI10$46+20%$17 – $83
Georgia GA109$44+16%$29 – $91
Oregon OR49$44+15%$17 – $62
Idaho ID24$41+8%$14 – $89
Wyoming WY16$39+3%$22 – $57
Alabama AL45$380%$12 – $77
Ohio OH141$37−4%$26 – $77
Montana MT33$36−5%$17 – $73
Connecticut CT20$35−8%$18 – $56
Kentucky KY69$32−17%$13 – $70
Tennessee TN81$30−22%$11 – $68
Indiana IN96$30−22%$19 – $59
Missouri MO76$28−25%$12 – $68
New York NY118$28−25%$12 – $90
North Carolina NC89$28−28%$18 – $124
Louisiana LA49$26−32%$8 – $47
West Virginia WV44$22−43%$14 – $64
Arkansas AR37$21−45%$14 – $44
Oklahoma OK74$20−46%$11 – $52
Utah UT43$17−55%$14 – $89
California CA148$16−58%$5 – $34
Michigan MI101$16−58%$8 – $47
Florida FL152$15−61%$7 – $40
Mississippi MS57$14−63%$8 – $55
New Jersey NJ57$13−65%$13 – $57
Delaware DE7not enough hospitals filed a usable price

Hospitals like yours

The same distribution cut by the kind of hospital. Hospital type and ownership come from CMS; a hospital counts as part of a system when its price file is shared with at least one other hospital under the same brand. The national median is $38.

Cash price by hospital segment. A segment needs at least 20 hospitals to show a figure.
SegmentHospitalsMedian20th–80th
Hospital type
Acute care2,303$32$13 – $82
Children’s64$38$19 – $101
Critical access979$53$20 – $103
Ownership
For-profit467$42$14 – $116
Government633$43$17 – $97
Non-profit2,246$36$14 – $87
System affiliation
Part of a system2,718$37$14 – $91
Independent638$42$18 – $92

Cash price vs list price

Median list price (chargemaster)$60
Median cash price$38
Cash as a share of list, median hospital65%

At the typical hospital, asking for the cash price cuts the list price by about 35%. The share is the median of each hospital’s own cash ÷ list ratio, over hospitals that filed both — not the ratio of the two medians above.

The same procedure, inpatient

2,272 hospitals filed a usable inpatient cash price for this code. The median is $38 and the average of the middle 60% is $42; most fall between $15 and $89.

Cash price percentiles, inpatient, across 2,272 hospitals. Each figure is a price a hospital filed. 10th: one in ten hospitals charges less; 25th: a quarter charge less; Median: half charge less; 75th: three quarters charge less; 90th: nine in ten charge less.
10th25thMedian75th90th
$10$18$38$75$124

The most expensive tenth of hospitals charges at least 12× what the cheapest tenth charges ($10 vs $124).

Of the 2,498 hospitals that filed this code inpatient, 2,272 gave a usable cash price; 115 carried a plausibility flag, 0 filed a number their own filing marks as not a price, 0 gave an estimate, and 111 a list price only.

How complete is this

3,356 of the 3,956 hospitals with a price file in our corpus (85%) filed a usable outpatient cash price for this code, and those are the hospitals every figure on this page is computed from.

What the 3,654 hospitals that filed this code at all gave us
Usable cash price — counted3,356
Cash price carrying a plausibility flag — shown on the compare tool, excluded here161
A number the filing itself marks as not a price (a placeholder, a ratio) — excluded0
An estimate rather than a filed cash price — excluded1
A list price only, no cash figure — excluded136
Hospitals that filed this code3,654

Sections not shown for this setting

  • Against Medicare: Medicare has no separately payable national outpatient (OPPS) rate for this code, so there is nothing honest to compare against.
  • Since our last snapshot: there is no earlier snapshot at least 90 days older than this one yet.

We last checked these hospitals’ price files for changes between September 4, 2026 and September 25, 2026; the figures were computed September 25, 2026 from filings loaded through September 25, 2026. The percentiles and averages use only the “counted” row.

Related procedures

Part of the Laboratory - Chemistry and Hematology family.

Hospitals file this code under descriptions such as HC Acetylch Rec Block Ab(Achrp).

Method

Source
The machine-readable price files every U.S. hospital is required by CMS to publish, parsed per hospital. Nothing is surveyed, sampled or modelled.
What counts
One cash (self-pay) price per hospital for this code and setting. A hospital’s own filing can rule its number out — a placeholder, a “not applicable” note, a list price standing in for cash — and our plausibility checks can flag a price far below what every other hospital files. Both kinds are disclosed above and excluded from the statistics; on the compare tool a flagged price is still shown.
Statistics
Percentiles are discrete: each is a price some hospital filed. The average is the mean of the prices between the 20th and 80th percentiles. We never publish the plain mean, the minimum or the maximum. A state needs 10 hospitals and a segment 20 to show a figure; otherwise the row shows its count and no number. Medicare is the national unadjusted OPPS rate where one exists.
Names
Procedure names on these pages are our own plain-language titles, not the AMA’s copyrighted CPT descriptors. The code is the identity.

The full rules — sources, statistics, thresholds, limitations — are on the methodology page.

Download this page’s figures: JSON · CSV. Aggregates only — the per-hospital prices are on the compare tool.

Cite: HospitalRates.com, “Immunoassay for one substance, multiple-step method (CPT 83516) — cash price report,” computed September 25, 2026. https://hospitalrates.com/procedures/cpt-83516-immunoassay-for-one-substance-multiple-step-method